Effect of diet containing olive oil on theexpression of mir373 and LncRNA H19 as colorectal cancertumor factors

Background: Association between the diet and the incidenceof colorectal cancer is confirmed by several scientific reports.Identification of the pathways that diet factors can affect the tumordevelopment, progression or inhibition is the vital point intumor management. In this study we focused on the mir373 andLncRNA H19 expression as two well defined factors involvedin colorectal cancer in the mice fed by olive oil.Material and methods: 20 male mice were divided to 2 groupsand fed with standard food (N=10 as control group) and standardfood plus 500μL olive oil by NG ((N=10 as treatment group)for two months. In the next step, the animals were anesthetizedand a part of the colon and rectum of them was removed. TotalRNA was extracted and cDNA synthesis was performed. Finally,expression of mir373 and LncRNA H19 was quantifiedin relative method. Cytoscape software applied for design of thegene web including the studied molecules.Results: In this study, we detected about 4fold decrease inthe level of LncRNA H19 (P≤0.05, Sig.=0.000, FC=0.231).Expression change of mir373 was not significant (FC=0.928,P≤0.05, Sig.=0.364) compared to the control group. Pathwaydesign confirmed the results of LncRNA H19. It seem that theexpression changes of mir373 is correlate with the other stagesof colorectal cancer.Conclusion: Our findings demonstrate that olive oil can inhibitthe LncRNA H19 gene expression that is involved in the primarystages of colorectal cancer development, however, oliveoil has no significant effect on the expression of mir373. Thisresults is supported by previous reports about the role of mir373in progress of colorectal cancer to the metastasis

کلیدواژه های Effect of diet containing olive oil on theexpression of mir373 and LncRNA H19 as colorectal cancertumor factors:

Colorectal Cancer ، mir373 ، LncRNA H19 ، gene expression ، diet