Mass Cultivation Phytoplankton Nannochloropsis oculata in the indoor and outdoor systems

This study was carried out to culture Nannochloropsis oculata marine algae at Gomishan workshop located in Golestan province in the autumn and winter. The algae were transferred from Kuwait to research center of Khozestan province to culture and feed the marine fish culture. The culture was established in an experimental –scale at indoor 250ml at 20 liter tanks in liquid cultivated medium f/2 (45×106 cells ml-1) and outdoor at an average density of 16×106 cells ml-1 in liquid cultivation medium TMRL. Water was directly pumped from a nearby shore and filtered by filtration systems and was used to produce liquid cultivated medium. No significant difference in temperature, pH, salinity, light or dissolved oxygen (DO) were observed between treatments. The temperature in all the process was 25±2˚C and algal light intensity was above 2500±350 lux and salinity was 30-32 ppt. Aeration was provided from air blower pumps and transferred with PVC pipe. Rotifers (Brachionus plicatilis) mono culture was maintained in 500 liters out door tanks which were to be fed by Striped Mullet larvae. The size (15-20μ) and cells wall structure of Nannochloropsis oculata in compartion with chlorellas algae cell wall are very suitable to feed and digestion for Striped Mullet larvae.In other words , the high amount of polyunsaturated fatty acids (PUFA)in this algae is most essential for all the marine fish larvae. Acording to Sethi et al. (2004) stability of larvae during the culture in compartion with other scientific reports and results of this project in the past years was very good, which shows a positive role of these algae to feed the Striped Mullet larvae.

کلیدواژه ها:

Nannochloropsis oculata ، Mass Cultivation ، indoor ، Outdoor ، Iran