Effect of Carbamazepine and Oxcarbazapine on chromatin quality, Malondialdehyde and parameters in sperm of epileptic-mice

Background: Consumption of anti-epilepsy drugs, recently, has become very common. These drugs have a clear effect on the reproductive system.Objective: The study is aimed to assess the effects of carbamazepine (CBZ) and ox-carbazepin (OXC) on sperm parameters, chromatin quality, and DNA integrity and evaluate Malondialdehyde (MDA) in NMRI/ mice spermatozoa.Materials and Methods: Thirty-two adult male NMRI mice were separated into four groups. Group1 (Control), received basal diet; group 2, pentylenetetrazol (PTZ) (35 mg/kg); group 3, PTZ (35 mg/kg) and CBZ (30 mg/kg); group 4, PTZ (35 mg/kg) and OXC (15 mg/kg). CBZ and OXC were administered by oral gavage and PTZ was given as intra peritoneal (IP) method every 48 hr for 35 days. Finally caudal epididymis was removed, and placed in 1 mL Ham’ sF10 medium at 37oC for 15 min in incubator and then analysis of sperm parameters was executed. To asses sperm chromatin quality and DNA integrity, we use toluidine blue (TB), chromomycin A3 (CMA3), Aniline blue (AB) and TUNLE, and to observe lipid peroxidation and total antioxidant capability, the MDA were measured.Results: The mean sperm parameters in the epileptic group, were significantly lower than the other groups. The sperm parameters in the groups treated with OXC and CBZ drugs were distinctly better than the epilepsy group and weaker than the control group, (p<0.05). In sperm chromatin quality and DNA integrity’ s test there were no significant different between group CBZ and OXC in AB, TB, CMA3, TUNLE, (p<0.05). But there were a significant different between groups CBZ and OXC with group PTZ, (p<0.05). There were a significant different between groups CBZ and OXC with control group in AB and TB test. MDA tests showed a significant difference between CBZ and OXC groups, and a significant different between CBZ and OXC groups with control and PTZ groups, (p<0.05).Conclusion: The results of this study showed that epilepsy can weaken sperm parameters, which can be leading to the increased production of reactive oxygen species (ROS). The use of antiepileptic drugs slightly improves the parameters and the quality of the chromatin and DNA integrity of the sperm epileptic mice and the lipid peroxidation in this group.

کلیدواژه ها:

Carbamazepine ، Oxcarbazepine ، Sperm chromatin ، Malondialdehyde ، Mice