Sperm DNA damage have an effects on embryo aneuploidy in ICSI-CGH array cycles

Background: There is clinical evidence to show that sperm DNA damage could be a marker of sperm quality and extensive data exist on the relationship between DNA damage and male fertility status. Sperm DNA fragmentation can be the most common reason for the transmission of anomalies of the father s DNA to a child that seen in a high percentage of sperms in infertile men.Objective: To investigate the effect of sperm DNA damage on embryo aneuploidy in ICSI-CGH array cycles.Materials and Methods: In this study, 40 patients with recurrent implantation failure (RIF) were selected; they had at least 3 times of good quality embryo transfer with failure in the implantation. both women and men appeared to have no problem. All women were between 25-35 and stimulated with GnRH agonist and oocyte isolation was performed after follicles were picked up. Semen samples were also analyzed and DFI measured using TUNEL by flow cytometry. According to their TUNEL results, 2 groups were defined, 1: DFI > 20% and 2: DFI <20%. Intra-cytoplasmic sperm injection was also performed and then the day 3 embryos were subjected to blastomere biopsy and evaluated by CGH array (Genomic Comparative Hybridization). Semen parameters, TAC, ROS and malondialdehyde (MDA) formation were analyzed between both groups also the correlation between embryo aneuploidy and semen parameters were evaluated.Results: The results of this study showed that sperm with high DFI (DFI > 20) significantly increased the number of the aneuploidy embryo than sperm with low DFI (p<0.001). Also, by increasing the DFI, the level of MDA significantly increased (p<0.001). The level of ROS and TAC were increased and decreased respectively but this was not significant by DFI (p> 0.001).Conclusion: These data indicate that sperm DNA damage have a significant effect on embryo chromosome aneuploidy. So, Embryo selection by aCGH should be considered in couples with high DNA fragmentation.

کلیدواژه ها:

Aneuploidy ، DNA fragmentation ، Malondialdehyde ، TUNEL