Molecular phylogeny of section Dendrobrychis (Onobrychis) based on cpDNA rpl32-trnL UAG sequences

The genus Onobrychis is one of the difficult genera in Fabaceae, which dispersed in Anatolia, Iran, and the Caucasus. Onobrychis in Flora Iranica have more than 82 species that 61species grow in Iran and at least 27 species are endemic. All species of the genus have one segmented legume. Onobrychis in Flora Iranica divided into two subgenera. The section Dendrobrychis is in Onobrychis subgenus. The section has seven species in Flora Iranica area of which O. cornuta have two subspecies and O. afghanica have three subspecies. For phylogenetic relationships between them, 12 taxa of this section and 3 taxa as outgroup included in this study. The rpl32-trnL UAG region amplified through PCR by appropriate primers. Then sequenced and sequences aligned by MUSCLE software and followed by manual adjustment. Maximum parsimony and Bayesian methods were applied. 12 best tree with 85 steps, CI=0.859, RI=0.906 and HI=0.141 were generated. As result, the members of the section formed a monophyletic group with high support. O. arnacantha and O. echidna were sisters; O. elymaitica is nested within the clade correspond to the subspecies O. cornuta. Within the clade O. cornuta subsp. Leptacantha has a close relationship with a subclade belong to O. cornuta subsp. cornuta of Kerman and Azerbaijan provinces

کلیدواژه ها:

Molecular phylogeny ، Dendrobrychis ، Onobrychis