Improved cytotoxic effect of liposomal cinnamaldehyde on MDA-MB-231 breast cancer cell line

Presently, the emergence of the herbal liposomal formulation as a kind of drug delivery system in cancer treatment is developed. Cinnamaldehyde (CA) as a natural phytochemical show potentially antiproliferative effect in cancer cells. Cinnamaldehyde encapsulated in a liposome to study its anticancer properties compared with free cinnamaldehyde on MDA-MB-231 breast cancer cells. In this way, liposomal cinnamaldehyde was prepared by thin film hydration technique. The formulation is characterized by particle size, zeta potential, scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR). MTT, flow cytometric assay and DAPI staining was applied to recognize the apoptotic rate and molecular mechanism, respectively. The liposomal cinnamaldehyde indicated average size of 59.64 nm with encapsulation efficiencies of > 80%. The IC50 values for free cinnamaldehyde and liposomal cinnamaldehyde were estimated 43.61ppm and 29.52ppm, respectively. So our results demonstrated that encapsulation of CA increases the cytotoxic effect of CA. Remarkably, encapsulated CA leads to a decrease in the proliferative percentage. This research proposes that encapsulation of cinnamaldehyde significantly increases the anticancer effect in comparison with free CA

کلیدواژه ها:

Cinnamaldehyde ، Drug-delivery ، Apoptosis ، Liposome