Rat bone marrow-derived mesenchymal stem cells can potentially decrease the hTERT gene expression in chronic myeloid leukemia cell line

Chronic myeloid leukemia (CML) is a hematopoietic stem cell-derived and progenitor-driven myeloproliferative disorder that may rise from a clinically manageable chronic phase (CP) to an incurable blastic phase. As is the case in all cancers, telomerase activity and hTERT gene expression play an important role in the progression of CML. A significant increase in hTERT gene expression has been reported in each of the three phases of CML, and this enhancement is accentuated during the progression of the disease. The multi-lineage nature of CML suggests that stem cells are important targets of this disease. Therefore, the aim of this study is to investigate the effect of BMSCs on the hTERT gene expression of the co-cultured K562 cell line. In this study, the bone marrow of the adult Rattus norvegicus was collected, mononuclear cells were separated by Ficoll Hypaque and bone marrow-derived mesenchymal stem cells (BMSCs) were isolated. In the following, the flow cytometry method, as well as RT-PCR, were performed to investigate the MSCs-surface markers. On the other word, K562 as chronic myeloid leukemia cell line were cultured in RPMI/1640. After reaching a confluency of cells, BMSCs co-cultured with K562 cell line for 7 days (1:10). At the end of co-culture time, K562 cell line was collected, total RNA was isolated, and cDNA was synthesized and subjected to Real-time PCR for investigating the hTERT gene expression. It was found that BMSCs had high levels of expression of CD44 (94.5%) and CD90 (87.1%) and hematopoietic cell lineage-specific antigens, such as CD31 (0.07%), and CD56 (0.9%) were not expressed in these cells. In addition, quantitative real-time PCR showed that BMSCs cause to significantly decrease the gene expression of hTERT. Taken together, the data showed that bone marrow-derived mesenchymal stem cells cause to decrease the hTERT gene expression of K562 cell line as a therapeutic approach.

کلیدواژه ها:

hTERT gene expression ، Bone marrow-derived mesenchymal stem cell ، Real-time PCR