P-glycoprotein gene expression in drug resistance breast cancer cells treated with benzylisoquinoline alkaloids
محل انتشار: نهمین کنگره بین المللی سرطان پستان
سال انتشار: 1392
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 329
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
این مقاله در بخشهای موضوعی زیر دسته بندی شده است:
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
ICBCMED09_095
تاریخ نمایه سازی: 29 فروردین 1397
چکیده مقاله:
Over-expression of P -glycoprotein (p-gp) in tumor cells is one of the most important mechanisms leading to multidrug resistance (MDR), which impairs the efficacy of chemotherapy by expelling a variety of lipophilic anti-cancer drugs. During a screening of MDR reversal agents among a family of benzylisoquinoline alkaloids some identified with moderate to strongly potent cytotoxicity in anti-cancer drug resistant subline, MCF-7/ADR, derived from MCF-7 cell line. Here we investigated the gene expression level of p-gp in both resistant and parental cells. MCF7/ADR and MCF7 were treated with different concentrations of the pure natural alkaloids for 48 h, the resistant cells in presence of doxorubicin. Total RNA extracted from each sample was converted to cDNA by reverse transcriptase and p-gp copy numbers were quantified by real -time Rotogene technology using. Expression levels were normalized relative to the transcription level of β2 -microglobulin as a housekeeping gene. After 48 hours treatment by some of the alkaloids, expression of the target gene was down -regulated considerably and in concentration dependent manner. These results suggest that those alkaloids with stronger cytotoxicity effects on resistant cells can reverse MDR by multiple mechanisms at least in part through down regulation of p-gp expression. In conclusion natural alkaloids are valuable compounds to investigate their effectiveness against drug-resistance in more detail and promising start point to develop new drugs.
کلیدواژه ها:
نویسندگان
z Jarrahifar
Dept. Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
s Kazemi Noureini
Dept. Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran