Comparative study of amyloidogenic potential of AgNO3 and Freund s Adjuvant (AF) with that of Vitamin Free Casein, on Spatio-Temporal Pattern of Experimental Amyloidosis in Mice

Reactive amyloidosis is a condition that complicates a long list of chronic inflammation, chronic infectious, malignant, and hereditary disorders. In the present study the potential effects of two amyloidogenic substances: ie. AgNO3 and Freund s Adjuvant (AF) with that of vitamin free casein, on spatio-temporal pattern of experimental amyloidosis in mice were compared. For this purpose atotal of 40 male Swees mice, obtained from Pasteur Institute Tehran, after being weighted were randomly divided into 4 groups including 2 treatments, 1 control (vitamin free casein) and 1 positive control (normal saline). At the end of 3rd, 5th and 7th weeks of experiment 3 mice were randomly selected and euthnised. Spleen sample of each animal obtained and preserved in 10% neutral bufferformalin. Sample were then processed through different stages of dehydration, clearing and impregnation and finally embedded in paraffin blocks. Sections of 5μm thickness then cut and stained by alkaline Congo red techniques. Spleen weights and the data obtained from microscopic quantitative analysis did show no significant differences between groups Aand B, Aand C, and B and C.But significant differences were observed between groups Aand D, B and D, and C and D respectively. It is concluded that two compounds ie; AgNO3 and Freund s Adjuvant have the same potential, as does vitamin free casein have, in spatio - temporal pattern of experimental amyloidosis in mice. Islamic.Azad.Univ., Garmsar Branch. 5,1:73-77,2010 .

کلیدواژه ها:

amyloid ، AgNO3 ، Freund s Adjuvant ، vitamin free ، mice