THE EFFECT OF DIFFERENT STRAINS OF AGROBACTERIUM RHIZOGENES AND TYPES OF EXPLANT IN CREATION OF HAIRY ROOTS FOR HYOSCYAMUS RETICULATUS
محل انتشار: مجله بین المللی مطالعات علمی، دوره: 1، شماره: 2
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 859
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شناسه ملی سند علمی:
JR_IJSS-1-2_011
تاریخ نمایه سازی: 9 خرداد 1396
چکیده مقاله:
The use of bacterium Agrobacterium rhizogenes for hairy roots induction in medicinal plants species of due to increased amounts of metabolites, high proliferation rate of interest is located. We investigate the optimization of hairy root cultures Hyoscyamus reticulatus plant showed that strains of bacteria and explant type effectively transformed explants on impulse. An MSU strain has the greatest impact on transformation and the second effective strains were A7 and D7. Hypocotyl and cotyledon explants of creating transgenic plants showed more effective.
نویسندگان
Bardia Moradi
Horticultural Science PhD student of Islamic Azad University, Science and Research Branch, Tehran
Bahram Delfan
Lorestan University of Medical Sciences
Peyman Hashemi
Lorestan University · Department of Chemistry
Amir Mousavi
National Institute of Genetic Engineering and Biotechnology (NIGEB)