THE EFFECT OF DIFFERENT STRAINS OF AGROBACTERIUM RHIZOGENES AND TYPES OF EXPLANT IN CREATION OF HAIRY ROOTS FOR HYOSCYAMUS RETICULATUS

سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 859

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شناسه ملی سند علمی:

JR_IJSS-1-2_011

تاریخ نمایه سازی: 9 خرداد 1396

چکیده مقاله:

The use of bacterium Agrobacterium rhizogenes for hairy roots induction in medicinal plants species of due to increased amounts of metabolites, high proliferation rate of interest is located. We investigate the optimization of hairy root cultures Hyoscyamus reticulatus plant showed that strains of bacteria and explant type effectively transformed explants on impulse. An MSU strain has the greatest impact on transformation and the second effective strains were A7 and D7. Hypocotyl and cotyledon explants of creating transgenic plants showed more effective.

نویسندگان

Bardia Moradi

Horticultural Science PhD student of Islamic Azad University, Science and Research Branch, Tehran

Bahram Delfan

Lorestan University of Medical Sciences

Peyman Hashemi

Lorestan University · Department of Chemistry

Amir Mousavi

National Institute of Genetic Engineering and Biotechnology (NIGEB)