Enhanced skeletal muscle maintenance on bioinspired Ponytail palm-derived ۳D matrices treated with whey protein

Skeletal muscle is most abundant tissue with critical important for optimal metabolic homeostasis in the human body that support movement, postural behavior, and breathing. Increasing rates of muscle injuries due to accidents, degenerative diseases or sports led to the movement for finding a bioinspired supportive scaffolds for muscle regeneration. Exploiting natural nanoarchitecture has recently suggested as biocompatible, non-toxic and low-cost approach to address such issues. Here, C۲C۱۲ cells were cultured on decellularized Ponytail palm treated with whey protein to investigate the capability of natural patterned cellulosic matrices on improving proliferation and differentiation of skeletal muscle cell line confirmed by SEM, AFM, and FTIR as well as MTT assay, and DAPI staining. The cells exhibited increased adhesion, proliferation with better viability rates and maintenance on nanoarchitecture decellularized cellulose scaffolds without any toxicity while treating with whey protein supported the cell attachment, and differentiation of C۲C۱۲ cells. Altogether, ponytail palm-derived cellulosic scaffold can simulate the natural cellular niche for skeletal muscle tissue culture majorly when used with whey protein and thus it can be considered as promising sustainable biomaterial for human muscle tissue engineering and developmental studies.

کلیدواژه ها:

Tissue engineering ، bioinspired material ، scaffold ، whey protein