investigating the cytotoxic effects of synthesized green cerium oxide nanoparticles on glioma and normal fibroblast cells: separation of nanoparticles by a different and new method

Glioblastoma, as the most common and malignant type of brain tumor, is a big challenge for the health system. The main treatment is surgery, however, the prognosis of the tumor is very bad. Therefore, it is very necessary to investigate new and effective treatment methods. One of them is the targeted delivery of drugs with the help of nanoparticles. Recently, cerium oxide nanoparticles (CONPs) have gained much attention in biomedicine due to their distinct cytotoxicity and therapeutic potential. For the first time, this study performs the separation process of these nanoparticles using a different extract and then evaluates the antioxidant and prooxidant effects of green nanoparticles on normal and cancer cell lines. Materials and methods: CONPs were synthesized from flax seed extract (Linum usitatissimum L.) and characterized using UV-Vis, FT-IR, FESEM/PSA, EDAX and XRD techniques. MTT assay and DCFH-DA dye were used to measure cytotoxicity and intracellular reactive oxygen species (ROS) levels, respectively. Finally, by conducting bioinformatics studies, the possibility of the drug passing through the blood-brain barrier was investigated. Results: FESEM/PSA analysis showed spherical nanoparticles with an average size of ۵۲.۵۱ nm. FT-IR analysis showed that the synthesized CONPs have a pure and crystalline structure. CONPs showed no cytotoxic effect on normal human fibroblast (HFF) cells, while inhibiting the growth of glioblastoma multiforme (U۸۷) cells. Furthermore, CONPs significantly increased the intracellular ROS levels in U۸۷ cells compared to the control group. Also, bioinformatics analysis showed that this nanoparticle can effectively cross the blood-brain barrier. Conclusion: CONPs inhibited the growth of U۸۷ cancer cells by increasing ROS levels, while at the same concentration, they had no effect on intracellular ROS levels in HFF cells. Also, the ability of this nanoparticle to cross the blood-brain barrier was shown. This drug has the potential to be used in targeted drug delivery methods. Finally, this nanoparticle has significant anti-cancer effects and can be effective in the delivery of various drugs.

کلیدواژه ها:

Cerium oxide nanoparticles ، Glioblastoma ، Green synthesis method ، Reactive oxygen species