Transcriptional biomarkers for early detection of colorectal cancer

Colorectal cancer (CRC) ranks second in terms of cancer-related mortality and is the third most frequently diagnosed cancer. also known as bowel cancer, colon cancer, or rectal cancer, is the development of cancer from the colon or rectum (parts of the large intestine). Most colorectal cancers are due to lifestyle factors and genetic disorders. Risk factors include diet, obesity, smoking, and lack of physical activity. Dietary factors that increase the risk include red meat, processed meat, and alcohol. It typically starts as a benign tumor, often in the form of a polyp, which over time becomes cancerous. Colorectal cancer may be diagnosed by obtaining a sample of the colon during a sigmoidoscopy or colonoscopy. This is then followed by medical imaging to determine whether the disease has spread. It is more common in developed countries, where more than ۶۵% of cases are found. It is less common in women than men. Finding new hub genes that were beneficial for CRC prognosis and focused therapy was the aim. From the gene expression omnibus (GEO), GSE۴۴۰۷۶, GSE۲۱۵۱۰, GSE۹۳۴۸, GSE۸۶۷۱, and GSE۸۱۵۸۲ were filtered out. GEO۲R was used to identify differentially expressed genes (DEGs), which were shown to be enriched in the KEGG pathway and GO term in DAVID. Hub genes were eliminated during the construction and analysis of the PPI network using STRING. Utilizing the cancer genome atlas (TCGA) and genotype-tissue [removed]GTEx), GEPIA assessed the associations between hub genes and CRC prognoses. KIF۲۰A and TPX۲ were hub genes with excellent predictive value and substantially elevated mRNA levels in colorectal cancer. KIF۲۰A and TPX۲ were found to be strongly linked to transcription factors, miRNAs, and lymphocytes that infiltrate tumors, indicating their potential role in the control of colorectal cancer. The current study employed the Real-time qPCR to assess the accuracy of bioinformatics studies. The findings of the laboratory indicated that there is increased expression of KIF۲۰A and TPX۲ in CRC samples when compared to control samples.

کلیدواژه ها:

colorectal cancer ، Bioinformatic ، TPX۲ ، KIF۲۰A