Silibinin restrict cell survival and cell invasionin glioblastoma multiforme cells

Introduction: Glioblastoma multiforme (GBM) is the mostcommon and aggressive form of primary brain cancer in adultswith significant neurologic morbidity. Despite multimodalitytreatment regimens, GBM is associated with extremely poorprognosis and high mortality. The potential anti-neoplastic effectsof natural plant compounds have attracted widespreadinterest in cancer therapy. In this study we used silibinin, anaturally occurring flavonoid, to restrict cell proliferation andmigration of GBM cell line.Materials and Methods: In this study, we used U۸۷ cell line inRPMA۱۶۴۰ media with ۱۰% FBS. The microculture tetrazoliumtest and trypan blue exclusion assay were used to determinecell viability. We also investigated the restrictive potentials ofsilibinin on cell cycle progression, colony formation potential,and cell migration.Results: The results demonstrated that the silibinin treatmentrestrict cell proliferation in dose-dependent and time dependentmanners and also inhibited colony forming potentials. Flowcytometry analysis showed G۲/M arrest. Moreover, silibinintreatment led to reduction in cell invasion.Conclusion: The standard treatments cannot eliminate GBM.Taken together, this study indicate that silibinin could beemerging treatment modality against GBM. silibinin can alsoovercome the blood–brain barrier for effective treatment andshould be explored in clinical trials as possible therapeutic opportunityfor prolonging life of patients with GBM.

کلیدواژه ها:

Glioblastoma multiforme ، silibinin ، anti-cancer-compound ، cell