Polymorphisms effects on structure and function of OeACP۲ and OeACP۳ proteins

Olive oil has fundamental positive influences on human health and prevent cardiovascular diseases. Fattyacid biosynthesis (FAS) pathways produce and store lipids in mesocarp of olive (Olea europaea L.). Animportant cofactor of fatty acid biosynthesis is acyl carrier protein (ACP) that acts as a shuttle. It makescovalent binds with fatty acyl intermediates and moves them from previous enzyme to next enzyme of thepathway. So mutations in ACPs may affect and disrupt fatty acid biosynthesis and oil producing. ACP hasthree loci in plants that each of them has two alleles. OeACP۲ and OeACP۳ have more crucial role andlargely found in olive fruit and leave. Hence, we extracted DNA from two Iranian olive cultivars, Shengeand Mari, with low and high quality oil respectively. Then whole genome next generation sequencing (NGS)was done and mutations in these genes were found. We identified single nucleotide polymorphisms (SNPs),insertions and deletions (InDels) in DNA sequences and predicted tertiary structure of proteins with onlineservers such as I-TASSER and Robetta by homology modeling and ab initio methods. Finally we found someeffective polymorphisms that seem to impact on structure and function of acyl carrier proteins.

کلیدواژه ها:

olive oil ، FAS ، ACP ، polymorphism