Selection of blood biomarkers to diagnose therapeutic resistance in people with epilepsy

Epilepsy is a common brain disease affecting ۷۰ million people worldwide. Antiepileptic drugs dramaticallyprevent seizures in patients, but about one-third of patients are resistant to these drugs. Therefore, findingblood biomarkers helps to diagnose and treat patients. Research shows that mutations in genes encoding ionchannels play a role in epilepsy.LncRNAs play an important role in the central nervous system, withdysfunction and expression changes associated with epilepsy.Method: Initially, Array express and GEO databases were examined to identify and express gene changes inthis disease. Finally, the GSE۷۱۰۵۸ study, which is a type of RNA sequencing technology, was selected.According to the type of data, R software and related packages were used for analysis. After evaluation andchanges in gene expression, a list of genes and LncRNAs that had more than double and significantexpression changes (pvalue <۰.۰۵) were selected among the study groups. Because these ion channels playan important role in epilepsy, the list of genes related to ion channels was extracted using the HGNGOdatabase. Also, to identify lncRNAs related to ion channel genes, the correlation between them was evaluatedusing the Pearson correlation coefficient. At this stage, lncRNAs with a correlation coefficient greater than+۰.۷ or less - ۰.۷ and a significant level of pvalue <۰.۰۵ were selected.Results: According to the analyzes performed on the GSE۷۱۰۵۸ data, the hcn۱, hcn۲, hvcn۱ genes, whichare related to ion channels, and the LncRNAs of FOXN۳-AS۱ and SLC۲۶A۴-AS۱ have more than doublethe expression changes and have become.Conclusion: Considering the results of data analysis and changes in the expression of ion channel genes andrelated LncRNAs and their importance in causing epilepsy, it is suggested that changes in gene expressionbe studied in a detailed laboratory study.

کلیدواژه ها:

epilepsy ، ions channel ، biomarker