Investigating the differential expression of starch and transcription factor encoding genes and their relations in wheat cultivars with different bread quality at ۵ days post anthesis

Wheat starch is one of the most important sources of carbohydrates, since every study about the regulatingfactors of starch metabolism can improve starch quantity and quality. The aim of this research is studying therelation between differential expressed starch and transcription factor (TF) genes at ۵-days post anthesis inwheat cultivars with high (Pishtaz) and low (Navid) bread quality.The transcriptome of cultivars compared by RNA-Seq. and based on wheat reference genome. The resultshowed “Alpha-glucosidase”, “Endoglucanases” and “۱,۴-alpha-glucan branching enzyme” genes thatinvolved in starch metabolism had differential expression. In addition, “General transcription factor IIHsubunit ۵ (Tfb۵)”, “GTE۹” and “MADS box” TFs showed differential expression. The ۱۲۰۰ upstreamnucleotide of starch gene employed for searching cis-regulating motifs in Place and PlantCare databases. TheDNA binding motif of differential TF identified based on literature search. We found CArG box DNAbinding site for MADS box, HD۱ and HD۲ for Tfb۵ and transcriptionally competent chromatin binding forGTE۹. Alpha-glucosidase with lower and Endoglucanase with higher expressions in Pishtaz, have cis-actingelements motif for binding the Tfb۵. This TF contributes in transcription re-initiation, early elongation stepsand DNA repair. The reduced levels of Tfb۵ and Alpha-glucosidase can guide us to assume a regulating rolefor this TF on Alpha-glucosidase gene. ۱,۴-alpha-glucan branching enzyme and Endoglucanase have lowerand higher expression, respectively. We found a motif for binding the MADS box that actives in transcriptionactivating , regulation of flower, leaf, and root development , growth negative regulators, positive regulatorsof stress tolerance. The decrease in ۱,۴-alpha-glucan gene can be contributed to lower expression of MADSbox.It seems that MADS box and Tfb۵ negatively controls Endoglucanase expression, has not affinity forbinding to its upstream or this gene is not their target genes.

کلیدواژه ها:

Triticum aestivum ، Bromodomain ، cis-regulating ، Alpha-glucosidase ، Endoglucanase