Detection and evaluation of protein complexes affecting on the skeletal muscle of beef cattle

Protein complexes are a collection of proteins that interact at a specific time and site to perform a specificbiological process. proteins as single units cannot perform well, but what causes a particular phenotype is aset of protein interactions. one of the traits that has been considered in the livestock industry is skeletal musclegrowth and meat quality. growth traits are quantity variables that are controlled by many genes. the proposeof this study is the detection and study of protein complexes in protein-protein interaction networks relatedto beef skeletal muscle. gene expression data with GSE۲۵۵۵۴ access number were downloaded from GEOdatabase. These data are related to the muscle tissue of beef cattle. filters for identifying genes with differentand significant expression were (-۲> fold Chang> ۲) and (Adj P-Value <۰.۰۵). Which was done using theLimma package available in R software. Protein complexes were determined by the MCODE plugin inCytoscape software. In these complexes, the proteins with the highest communication score were squaredand introduced as seeds. DAVID database was used to identify the activity of seed genes. in the present study,۱۴۳ genes were examined, and three complexes were formed, in which the seed genes include FASN, FN۱,and SDHC. The results of gene activity showed that FN۱ gene has various roles including cell adhesion andcell growth. The FASN gene is involved in biological processes such as lipid transport, lipid metabolism andmuscle growth, it also affects the quality of meat. The SDHC gene is involved in the mitochondrial electrontransfer chain and is responsible for the transfer of electrons from succinate to coenzyme Q. Therefore, thesegenes can be introduced as the key genes that control the skeletal muscle growth of beef cattle.

کلیدواژه ها:

Protein Complex ، Muscle Growth ، PPI network