Transcriptome profiling analysis of Suaeda salsa shoots under salinity stress

Salinity is one of the consequential abiotic stresses that significantly impact agricultural yield worldwide.euhalophyte Suaeda salsa is a model plant that may help identify salt tolerance molecular mechanisms todevelop salt-resistant crops. In the present study, high-throughput RNA sequencing was conducted after theSuaeda salsa seedlings were exposed to ۰ mM, ۲۰۰ mM, ۴۰۰ mM, and ۸۰۰ mM NaCl for ۳۰ days. Thecomparative analysis of transcripts identified ۶۱۰, ۱۸۹۳, and ۱۶۹۱ genes significantly expressed at ۲۰۰mM,۴۰۰mM, and ۸۰۰ mM NaCl, compared to control, respectively. Gene Ontology (GO) and MapMan analysesrevealed that the differentially expressed genes were related to solute transport and nutrient uptake, proteinsynthesis, modification, hemostasis, transcriptional regulation, and Phytohormones action. Moreover, weidentified multiple genes associated with different transcription factor families, including ۱۴۳ DEGs, whichmay regulate salt stress-response in S. salsa. Finally, the DEGs in the salt response pathway were verified byQuantitative real-time PCR (qRT-PCR).

کلیدواژه ها:

Salinity ، Euhalophyte ، Suaeda salsa ، High-throughput RNA sequencing