Sox۲-Oct۴ Decoy Oligodeoxynucleotide−Encapsulated Niosome-zinc hybrid Nanocarriers: A Promising tool to suppress the Metastatic Properties of NTERA-۲ cancer stem-like cells

Introduction: Sox۲ and Oct۴ dysregulations could lead to increased cancer stem cells (CSCs) population in some tumor cells and resistance to common treatments. In this study, we investigated the synergistic effects of Sox۲-Oct۴ decoy oligodeoxynucleotides-encapsulated Niosome-zinc hybrid nanocarriers on X‐irradiation exposure as a combinational therapy on cancer-like stem cells. Methods: Because of the technical limitations, difficulties of keeping CSCs in culture, we used NTERA-۲ that known as cancer-like stem cell line. Sox۲-Oct۴ decoy oligodeoxynucleotides were designed based on Sox۲ gene promoter and synthesized. Physicochemical characteristics of ODNs-Encapsulated Niosome-zinc hybrid Nanocarriers (NISM@BSA-DEC-Zn) were investigated with FT-IR, DLS, FESEM and hemolysis assays. Further investigations were carried out using uptake, cell viability, apoptosis, cell cycle, and scratch repair tests. All above assays were performed at with and without X-rays (fractionated ۲Gy) exposure conditions. Results: Our results regarding physicochemical characteristics showed that the Niosome-Zn nanocarrier system was successfully synthesized. NISM@BSA-DEC-Zn was efficiently taken up by NTERA-۲ cells and significantly inhibited cell growth, reduced cell migration, and increased apoptosis in both with and without X-rays exposure conditions. Furthermore, NISM@BSA-DEC-Zn treatment resulted in G۱ and G۲/M cell cycle arrest in without and with radiation exposure, respectively. Conclusion: The results provided by the current study suggest that the presented nanocarrier system can be a promising tool for targeted drug delivery in cancer therapy and the application of decoy strategy could increase the sensitivity of cancer cells toward irradiation, which has a potential to eliminate the cancerous cells from tumors in the form of combination therapies.

کلیدواژه ها:

Cancer stem cells ، Decoy oligodeoxynucleotide ، Sox۲-Oct۴ ، Radiotherapy ، Anticancer effects