Impact of vitamin D on TGF-ᵦ Expression in peripheral Blood Mononuclear Cells of asthmatic patients

Considering the importance of the immune system in increasing the pathogenesis of asthma through the expression of TGF-β gene, the regulatory effect of vitamin D on the expression of this gene in the culture medium of mononuclear cells of patients with asthma was investigated. For this purpose, ۲ groups of ۲۰ of both sexes were selected as the case group )asthmatic patients) with a mean age of ۲۷/۶۴±۵/۱۲ and the control group (healthy people) with a mean age of ۲۸/۱۷±۴/۶۴ in Jahrom city. ۵ cc of blood was taken from both groups, and after heparinization and dilution one by one with PBS, it was added to the follicle solution and centrifuged for ۲۰ minutes. The isolated peripheral mononuclear cells were cultured in medium (RPMI + FBS). The cells were divided in control group (without treatment), the two treatment with vitamin D at concentrations of ۱۰-۶ and ۱۰-۷M and the group treated with dexamethasone (as positive control). After RNA extraction, their cDNA was made and the expression of TGF-β gene was measured and compared in case and control groups using real-time PCR technique. Obtained data were analyzed with Graph pad statistical software (۲۰) at P <۰.۰۵. The results showed a significant decrease in TGF-β gene expression in cells treated with vitamin D and dexamethasone in the control group compared with the case group. There was no significant difference between the vitamin D-treated groups together and with the dexamethasone-treated group. Obtained findings indicate a positive effect of vitamin D in reducing asthma symptoms. So, with more research on the mechanism of this vitamin, it can be suggested as a potential factor in the prevention and management of asthma.

کلیدواژه ها:

Antigen stimulation ، Cytokine ، peripheral blood