Evaluation of the effect of coenzyme Q۱۰ on TFAM gene expression and the number of mt DNA copies of mice preantral follicles during in vitro culture

One of the best strategies to maintain fertility in infertile patients is in vitro maturation of follicles and eggs. In this study, ۱۴-day-old female NMRI mice used. Female mice are first sacrificed and the ovaries removed, the follicles removed mechanically and cultured in incubator for ۱۲ days. The follicles are divided into two groups of control and culture treatment in the presence of ۵۰ μM coenzyme Q۱۰.The study was performed in two parts. ۱) Developmental evaluation of preantral follicles; ۲) Molecular evaluation )Real time PCR ) of preantral follicles and granulosa cells. The diameter of preantral follicles in treatment group versus control was ۳۲۸.۴۱ ± ۷.۶۷ and ۲۹۹.۲۷ ± ۱۱.۵۵ respectively, which increased significantly in treatment group. TFAM expression in granulosa cells of treatment group versus control was ۱.۴۶ ± ۰.۱۰ and ۱.۰۲ ± ۰.۰۷ respectively, which increased significantly in treatment group. The expression of TFAM in oocytes of treatment group versus control was ۱.۸۱ ± ۰.۱۳ and ۱.۱۸ ± ۰.۱۸ respectively, which increased significantly in treatment group. The number of mt DNA copies of granulosa cells in treatment group versus control was ۴۸۴۵۸۹ ± ۱۰۹۷۹.۹۸ and ۳۱۴۷۸۶ ± ۷۲۰۷.۹۶ respectively, which increased significantly in treatment group. Coenzyme Q۱۰ by improving the expression of TFAM and increasing number of mt DNA copies and improving the mitochondrial function of preantral follicles leads to improved follicular development and growth.

کلیدواژه ها:

Follicle culture ، Development ، Mitochondria