Extraction and Investigation some of Biological Characteristics of Venom Rhopilema nomadica in the Persian Gulf

Jellyfishesarethe species of invertebratesbelong toCnidaria’sclass. The cnidocyte cells as prey hunter system of jellyfishes are located on their arms, around the mouth or other parts of body. Each bitingcell contains a corneous nematocyte and a capsule containing a spring hollow filament, andproper stimulator cause the nematocyte to be thrown abruptly. There are numerous reports of victims by different kind of jellyfishes.The sign and symptoms such as skin redness, shortness of breath, low blood pressure and sometimes death occurs fallowing jellyfishes bite. Therefore, the venoms ofjellyfishes are interestedby scientists in pharmaceutical research.The aim of this survey was to optimizethe method of venom extraction and study of some biological aspects of venom fromRhopilemanomadicaspecies such as total protein, molecular weight of venom components and toxicity (LD50).The samples were collected from Persian Gulf (around Qeshm Island) andtransferred to laboratory,then their tantacules were separated andwere frozen in -20 Co. The frozen tantacules were lyophilized.The venom extraction was performedbyfreeze& thaw method. Thetotal protein was 1.2 mg / ml, by Brad-Ford assay. SDS-PAGE detected three protein components with 170, 130 and 53 KDa, respectively.The toxicity test (LD50) was performed onBalb-c mice via intradermal (ID) rout and resulted 32μg/kg.Development of research on biological aspects of this jellyfish venom can prepared essential knowledge for future studies such as antivenom production and clear approaches to cure victims due tojellyfishes bites.

کلیدواژه ها:

Jellyfish ، Nematocytes ، Venom ، Rhopilemanomadica