شناسایی جدایه های ویروس تریستزای مرکبات به روش ‭RT-PCR ‬در استان مازندران

Citrus tristeza virus is one of the most harmful diseases in citrus plantings in the world. According to biological characters CTV has different strains. There is citrus tristeza disease in region and transmits by vectors in Mahdasht gardens. Therefore it is necessary to identify CTV strains in north of Iran. In this study RT-PCR method was used. In late studies 6 strains of CTV were reported in North of Iran and their major coat protein was amplified by IC-RT-PCR. After cloning and sequencing a product with 672 nucleotides was obtained. Nucleic acid and amino acid analyzing showed that 4 isolates are very similar and two of them are different. The most similarity of some isolates is with Japanese isolate (NuagA) and Korean isolate (Chenju). One of the isolates likes California strain (SY538). TN1 strain is different from the others. Keywords: Tristeza virus, Strains, Citrus and RT-PCR