Effect of probiotics mixture on memory function, inflammation markers and oxidative stress in Alzheimer s disease rats

Background and Aim : The most important pathogenesis of Alzheimer disease (AD) is linked to Aβ deposition, inflammation markers and oxidative stress. Probiotics are microorganisms that are believed to be benefits to human and animals. Probiotics reduced some oxidative stress biomarkers and inflammatory cytokine in some cases. For this reason the aim of this study was to determine effects of probiotics mixture on biomarkers of oxidative stress and inflammatory factors in AD model rats.Methods : In this study 50 rats were randomly divided to 5 group: control group, sham group , control+rivastigmine, AD group injected the Aβ1-40 intra-hippocampal and the AD+probiotics group, which was injected Aβ1-40 intra-hippocampal and 2ml (1010 CFU) probiotics (Lactobacillus reuteri ,Lactobacillus rhamnosus, Bifidobacterium infantis) orally once a day for 10 weeks. Memory and learning were measured by Morris water maze, for detection of beta-amyloid plaque, we used congored staining, oxidative stress measured by malondialdehyde level (MDA) and superoxide dismutase activity (SOD) and for assessment of inflammation marker (IL-1β and TNF-α) in the hippocampus, used ELISA kits.Results : . In treatment group spatial memory significantly was improved measured by Morris water maze. Probiotic administration decreased beta amyloid plaques in AD brain rats. MDA decreased and SOD increased in treatment group. Finally our result showed that probiotics reduced IL-1 and TNF- as inflammation markers that have important role in AD.Conclusion : This study showed probiotics could improve memory deficit, decrease beta amyloids formation, inflammation and oxidative stress in Alzheimer model.

کلیدواژه ها:

Alzheimer ، probiotic ، inflammation ، stress oxidative