Therapeutical effects of saffron on hippocampal BDNF and TNFα in experimental Multiple sclerosis in ovariectomized rat

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 278

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شناسه ملی سند علمی:

NSCMED08_281

تاریخ نمایه سازی: 15 دی 1398

چکیده مقاله:

Background and Aim : Multiple sclerosis (MS) is a complex autoimmune disorder which characterized by demyelination and axonal loss in the central nervous system (CNS). Crocus sativus L. (saffron) is an herb that has anti-inflammatory effects. The anti-inflammatory properties of estrogens was investigated. The aim of present study was the treatment effect of ethanol extract of saffron on hippocampal tumor necrosis factor alpha (TNFα) and brain derived neurotrophic factor (BDNF) in the experimental autoimmune encephalomyelitis (EAE) rat’s model of MSMethods : Thirty adult male Sprague Daweley rats were used into five groups. Sham (intra dentate gyrus (DG) injection of 3μl normal saline); treatment control (EAE induction without treatment); treatment 1 (saffron 60mg/kg/day for 21 days); treatment 2 (EAE induction + saffron 60mg/kg/day for 21 days) and treatment 3 (EAE induction + saffron 60mg/kg/day for 21 days+ estradiol 2μg/kg/day). Ovariectomy were done in all group except sham and treatment 1. Induction of EAE was done by 3 μl of 1% ethidium bromide injection in DG. TNFα and BDNF concentration was measured by ELISA kit. Data were analyzed by one way ANOVA and Tuckey as post-hoc test. Significant levels was considered P<0.05.Results : present data was shown that hippocampal BDNF in MS treatment control significantly was lower than sham, treatment 2 and treatment 3 groups, hippocampal BDNF in treatment two group was significantly (P<0.05) higher than another groups. In MS treatment control, treatment 2 and treatment 3 groups TNF was significantly (P<0.05) higher than sham groupConclusion : Present result were indicated that saffron improve the hippocampal BDNF and TNFα in MS without ovariectomy; therefore it has neuro-protective and anti-inflammatory effects. The effect of saffron in the ovariectomized rat with and without estradiol treatment has little effect. So it seems that saffron in present of intact ovary have beneficial effect; and in fact it need to not only estradiol but also all ovarian hormone for the best effect in MS

نویسندگان

Zahra Javid

Division of Physiology, Department of Basic Science - School of Veterinary Medicine - Shiraz University—Shiraz-Iran

Mahnaz Taherian Fard

shiraz univercity

Javad Sajedian Jard

shiraz univercity