Assessment of the circulating mir-218 expression level in under treatment epileptic patients as a noninvasive biomarker of PCOs

Background: Epilepsy is a common chronic neurologic condition with unknown mechanism. Moreover, side effects of antiepileptic drugs specially valproic acid (VPA) which is commonly used the treatment of epilepsy; is of interest. In women, VPA can also lead to androgenization, menstrual disturbances and PCOs. The incidence of polycystic ovary syndrome (PCO) increases in women with epilepsy (WWE).Objective: We aimed to define a non-invasive biomarker for early diagnosis of PCOs in WWE patients and to take preventative measures.Materials and Methods: The study was conducted on 15 WWE women at reproductive ages (20-40 yr) with PCOs, monotherapy with VPA and 15 women without PCOs. The women with history of diabetes, kidney and liver disease was excluded. After collecting patient ‘serum, total RNA were extracted. We use Q-RTPCR to identify the expressions level of miRNA-218 in epileptic patients with PCOs and without it.Results: In accordance with previous studies on PCOs and non-PCOs women, expressions level of miRNA-218 were significantly different between epileptic patients with pco and without it (p<0.05).Conclusion: Our results suggesting miRNA-218 as non-invasive biomarker to screen for drug side effects and prevent the progression of its complications. Furthermore, bioinformatics analysis indicate role miRNA-218 in Estrogen/ Adiponectin/ MAPK signaling pathway which may be of importance in PCOs.

کلیدواژه ها:

Epilepsy ، PCO ، Non-invasive biomarker ، miRNA-218