The frequency of rs14035 polymorphism of RAN gene and its association with unknown recurrent pregnancy loss in women referred to Yazd Reproductive Sciences Institute

Background: Spontaneous abortion occurs in 15% of clinical pregnancies before the 20th week of gestational age. Repeated pregnancy loss (RPL) is defined as two or more spontaneous abortion.Objective: It is indicated that genetic factors are responsible for 5% of RPLs. RAN gene is a member of the Ras superfamily. The mutation of RAN gene inhibits DNA synthesis and induces the altered gene expression in the uterus. Therefore RAN mutation may be involved in the mechanism of RPL. In this study, we assessed the prevalence of rs 14035 polymorphism of RAN gene in women with unknown RPL and also compared it with fertile women without the history of RPL.Materials and Methods: In the case-control study, 100 women with unknown RPL before the 20th wk of gestational age were recruited. The women were 20-40 yr old and their husbands were identified with normal karyotype in addition to normal sperm parameters. The control group included 100 women with regular menstrual cycles, who had at least one live birth and no history of abortion. The prevalence of rs 14035 polymorphism of RAN gene was investigated using PCR-RFLP technique.Results: Frequencies of the TT, TC, and CC genotype of RAN gene polymorphism in patients were 9%, 40%, and 51%, respectively while frequencies in control were 11%, 38%, and 51%, respectively. There was no significant differences between the genotypes in two groups (p=0.882).Conclusion: According to our study, rs14035 polymorphism of RAN gene appears to have no association with the risk of unknown recurrent pregnancy loss in the studied population. However, additional studies, especially with larger sample size are needed to validate these findings.

کلیدواژه ها:

RAN gene ، rs14035polymorphism ، RPL ، PCR-RFLP