Metabolome mapping of day 3 and 5 embryo culture medium using MALDI-TOF-MS

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 418

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شناسه ملی سند علمی:

RMED08_147

تاریخ نمایه سازی: 21 مرداد 1398

چکیده مقاله:

Background: The purpose of this study was to map secreted metabolome from embryo culture medium of Day 3 and 5.Objective: The purpose of this study was to map secreted metabolome from embryo culture medium of day 3 and 5.Materials and Methods: Embryo culture media was collected on day 3 and 5. Metabolome of pure culture medium (without embryo) and secreted metabolome of embryo culture medium Day 3 and 5 were extracted and analyzed by MALDI-TOF-MS. Metabolites were identified using The Human Metabolome Database-HMDB version 4.0. The Reactome Pathway Database was used for characterization of mapped metabolome of Day 3 and 5.Results: 775 m/z peaks were detected. More than 400 metabolites were identified of secreted metabolome from Day 3 and 5 embryo culture medium using Human Metabolome Database. However, only 9 identified metabolites were in overlap between Day 3 and 5. Reactome analysis showed metabolite belonging to metabolism, signal transduction and transport of small molecule were enriched. However, the majority of metabolites biological activity remains unknown.Conclusion: To the best of our knowledge this is the first metabolome mapping of embryo culture medium using MALDI-TOF-MS. Although, we mapped potential metabolome from Day 3 and 5 the function of majority of secreted identified metabolites remain unknown during embryo development. The potential embryo secreted metabolome from Day 3 and 5 can be found Supplementary material.

نویسندگان

N Ebrahimian

Reproductive Embryology and Andrology Research Center, Avicenna Research Institute, Academic Center for Education, Culture and Research, Tehran, Iran- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sc

MR Sadeghi

Reproductive Immunology Research Center, Avicenna Research Institute, Academic Center for Education, Culture and Research, Tehran, Iran

SM Kalantar

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

MA Khalili

Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran