Aneuploidy screening of arrested embryos derived from in-vitro matured oocytes

Background: High rates of aneuploidy have been reported either as adverse effects of in vitro manipulation or part of physiological in vivo development. However, there was no information on the rate of aneuploidy in IVM derived embryos that is one of the reasons for discarding immature oocytes in ICSI cycles. On the other hand, also, the association between embryo morphology and aneuploidy remains uncertain.Objective: This study aimed at evaluating aneuploidy and quality of arrested embryos derived from in-vivo and rescue in-vitro matured oocytes.Materials and Methods: In this study, 30 and 35 arrested cleavage stage embryos in case and control group were respectively analyzed by FISH technique for detecting aneuploidies in X, Y, 13, 15, 16, 18, 21 and 22 chromosomes.Results: There were insignificant differences between aneuploidy rates of embryos in both groups (p> 0.05), regarding to sex, autosome and total abnormalities also from the point of view chaotic and mosaicism. Moreover, chromosomal abnormality was observed in a high percentage of good quality embryos from both groups, without correlation between the morphological characteristics of embryos and chromosomal content.Conclusion: Our finding demonstrated neither embryo quality nor aneuploidy rate differ significantly between in vivo and in vitro derived embryo. Therefore, we would suggest IVM as a valuable and practical option for patients who have had to cancel IVF treatment cycles because of severe responses or resistance to routine hormonal therapies or those with low functional ovarian reserve.

کلیدواژه ها:

IVM ، Embryo Quality ، Aneuploidy ، FISH