Molecular identification of some endophytic fungi from Pterocarya fraxinifolia branches

Molecular identification of fungi was shifted to a new stage after PCR innovation. During the last 30 years, a huge of molecular data and sequences belonging to about50000 fungi were deposited in the world the biggest databases. These data helped mycologists to determine unknown species as soon as possible. Among different genomic regions introduced for molecular diagnosis of fungi, ribosomal internal transcribed spacers (ITS) regions are capable of accurate species determination. In the current research, after isolation, purification and microscopic studies of isolated fungi from branches of Pterocarya fraxinifolia were subjected to molecular and Bioinformatic surveys to determine their genus and species. Spacers regions between 18S and 28S loci contain 5.8S site were amplified with forward primer pairs ITS4 and ITS5. After sequencing of PCR products the harvested sequence were analyzed via BLAST of NCBI, then fungal genera and species were determined as Chaetomium globosum (100 %), Daldinia childiae (99%), Preussia minima(100%), Hypoxylon fuscum (99%), Lopadostoma fagi (99 %). PCR amplification and sequencing then bioinformatics analysis with BLAST is a quick and easy method for fungal identification. The ITS is a shortcut, which facilitates an unknown fungal diagnosis in comparison with classical and old morphological methods.

کلیدواژه ها:

Endophytic fungi ، Molecular identification ، ITS