Investigating the effect of plant growth regulators on the propagation of Mooseer (Allium hirtifolium Boiss.) in in vitro conditions

In order to determine the best hormone composition for in vitro multiplication of Khansar accession of Mooseer, an experiment was carried out in a factorial based on completely randomized design with different concentrations of BA (0, 2 and 4 mg/L) and NAA (0, 0.3 and 0.6 mg/L) in Murashig and Skoog medium. The basal plate of Mooseer was used as an explant. The results showed that the highest number of bulblet per explant (11.83) and the largest basal plate width (3.63 cm) were found in the culture medium containing 4 mg/l BA. The lowest number of bulblet per explant (4.53) and the minimum basal plate width (1.72 cm) were observed in a cultural medium without BA. Also, the highest percentage of callus production (51%) was found in the culture medium without BA. Results showed that the highest number of bulblet per explant was found in the culture medium containing 4 mg/l BA and 0.6 mg/l NAA

کلیدواژه ها:

Regeneration ، Tissue culture ، Basal plate ، Bulblet