A proteomics approach to identify metacyclogenesis regulated proteins in Iranian clinical isolates of Leishmania tropica

Leishmania as a protozoan parasite is the etiological agent of leishmaniasis, which is responsible for a spectrum of disease. Leishmania parasites including a dimorphic lifecycle are transmitted by the sand-fly vector. Promastigote forms in the alimentary tract of sand-fly are extracellularly flagellated. Metacyclogenesis defines as differentiation from procyclic to metacyclic promastigotes that correlates with high Leishmania infectivity. We compared the proteomes of procyclic and metacyclic promastigotes of L. tropica in Iranian isolates. Proteins of cell lysates were extracted and the protein concentration of stages determined by using BCA assay. After digestion of proteins with trypsin/LysC, Proteome profiling was done by LC-MS/MS. in the present study more than 100 proteins were identified in each understudy stages. Among these, 40S ribosomal protein SA, Proteasome endopeptidase complex, Putative serine peptidase and Mitochondrial RNA binding protein 1 were procyclic specific proteins. Tubulin beta chain and dynein light chain were detected in the metacyclic stage. This study has shown that a number of proteins differentially expressed in procyclic and metacyclic stages of L. tropica isolates. According to results, proteins associated with protein synthesis pathway were expressed in procyclic. Motion proteins were identified in a metacyclicstage in accordance with infectivity power of metacyclic form

کلیدواژه ها:

Leishmania tropica ، Proteomics ، LC-MS