Measurement of 17α-hydroxyprogesterone (17α-OHP) by using solid-phase extraction and HPLC method

Hydroxylase enzyme deficiency is one of the most common forms of congenital adrenal hyperplasia. Depending on the amount of enzyme deficiency, clinical symptoms may be severe or if the enzyme deficiency is not significant, the clinical symptoms would be manifested during puberty. The best way to diagnose this disease is to measure serum 17α-hydroxyprogesterone (17α-OHP) marker in infants blood. In this study, a new method for the detection of 17α-OHP metabolite has been introduced. For this purpose, the solid phase extraction method combined with liquid chromatography has been applied. First, the effective processes on the efficiency of the proposed method such as solvent type, sample volume, extraction solvent volume, and salt percentage were identified and optimized. Then, 17α-OHP was measured using HPLC. After optimization, the detection limit was 4 ng/ml and the measurement was achieved linearly in the range of 10 to 5000 ng/ml. The results indicate that this method can be used to measure the plasma concentration of the intended analyte in infants with congenital adrenal hyperplasia

کلیدواژه ها:

Congenital adrenal hyperplasia ، 17α-hydroxyprogesterone ، Solid phase extraction ، HPLC