Immobilization of laccase enzyme on Iron Oxide nanoparticle and determination of its activity

Immobilization of enzymes improves their properties for efficient utilization in various processes. Iron oxide nanoparticle is appropriate for different functions due to its valuable specification such as availability, inexpensiveness, nontoxicity, easy separation and high surface-to-volume ratio. In this study, iron oxide nanoparticle used in immobilization of laccase enzyme. This magnetic nanoparticle was prepared using coprecipitation method from inorganic salts of Fe (III) and Fe (II) with a stoichiometric ratio of 2:1 (Fe3+/Fe2+). This magnetic nanoparticle activated by 400 mM of NHydroxysuccinimide (NHS) and 20 mM of 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and eventually, laccase enzyme (0.025 g/ml) loaded into the solution pH of 5.5 and immobilization process accomplished by incubation lasting 24hours at room temperature.Under these optimum circumstances, laccase enzyme immobilized onto iron oxide nanoparticle by efficiency up to 90%.Overall, iron oxide nanoparticle is a proper support for immobilization of laccase and other enzymes

کلیدواژه ها:

Iron oxide ، Magnetic nanoparticle ، Enzyme immobilization ، Laccase