Design and fabrication a local surface plasmon resonance based on nanobioprobe in order to Shigella flexneri bacteria detection

Shigella flexneri bacteria is an important human pathogen which causes an acute bloody diarrhea known as shigellosis or bacillary dysentery. So, rapid detection of this pathogen in order to reduce the health risks of this pathogen is importance. Recently, biosensors due to a variety of reasons have replaced with many expensive diagnosis methods. In fact, gold nanoparticles due to high potential for conjugation with various biomacromolecules provide the suitable way in order to design the various nanobiosensors. Herein, an LSPR nanobioprobe has been developed based on gold nanoparticles-polyclonal antibody conjugated system in order to detect Shigella flexneri bacteria. In this study for the synthesis of nanobioprobe, specific polyclonal anti-Shigella protein has been conjugated at the surface of gold nanoparticles and this process has been confirmed by dynamic light scattering (DLS) measurement and observation of LSPR bond shift in UV/Vis spectrophotometer. After that, the sensitivity of the nanobioprobe was studied via monitoring the LSPR band alterations in the presence of different concentrations of Shigella Liquid medium (from 0-1000 CFU/ml). According to the results, the sensitivity of the nanobioprobe is about 100 CFU/ml whereas in comparison to the limit detection of ELISA tests is much more. So, LSPR based nanobioprobe has the probability of being applicated for the rapid diagnosis of the wide variety of pathogenic agents.

کلیدواژه ها:

Local surface plasmon resonance (LSPR) ، Nanobioprobe ، Gold nanoparticles ، Shigella flexneri