High-fat diet-induced renal tissue oxidative stress; the role of retinoic acid and atorvastatin

Obesity causes many problems such as cardiovascular and chronic kidney disease. The aim of this study was to evaluate the efficacy of co-administration of retinoic acid and atorvastatin in protecting the kidneys against damage induced by high-fat diet. Twenty-five male Wistar rats (200±20 g) were divided into five groups: 1) control (standard diet), 2) high-fat (cholesterol 1%, 75 days), 3) high-fat + atorvastatin (20 mg/kg per day, PO, on the 30th day, for 45 consecutive days), 4) high-fat + retinoic acid (5 mg/kg per day, PO, 45 days), and 5) high-fat + atorvastatin and retinoic acid. At the end, tissue sample was collected for biochemical total antioxidant capacity (TAC) and protein carbonyl (PCO) analyses. The results showed that atorvastatin – but not retinoic acid - increased the total antioxidant capacity (P<0.01) and decreased the protein carbonyl content (P<0.01) of the kidney tissue. Treatment with atorvastatin significantly mitigates high-fat dietinduced changes, probably due to its potent antioxidant and lipid-lowering effects. The effect of retinoic acid on renal protection in a high-fat diet is far less than that of atorvastatin. The protective effects of the combination of these two agents in the high-fat diet on the kidneys seem to be due to the effect of atorvastatin.

کلیدواژه ها:

High-Fat diet ، Retinoic acid ، Atorvastatin ، Antioxidant capacity ، Protein carbonyl