Valuation of H9N2 subtype of Avian Influenza tissue tropism in SPF chickens by virus isolation method kazeroun in city

Objective: Infections by Avian influenza virus (AVI) H9N2 subtype is a major cause of economic losses in Iranian poultry industry. This study was evaluated the tissue tropism of A/chicken/Iran/m.1/2010 (H9N2) virusMaterials & Methods:Tissue dissemination of A/chicken/Iran/m.1/2010 (H9N2) virus was investigated in different organs of SPF chicks. Forty two, dayold SPF chicks were divided randomly into two groups (21 chicks per group) in separate positive pressure isolators. At the age of 12days-old the chicks in group-1 were inoculated with 106 EID50 AIV by eye drop, group-2 was kept as the control group. The samples from various tissues were collected at 2,4,6,8,10, and 12 days post-inoculation (PI). The virus isolation methods was used for detection of the viruse dissemination.Results & Conclusion:In group-1 AIV was detected in the trachea, lungs, spleen and cloaca. Our results indicated that the virus was detected in trachea, lung and spleen samples of infected groups on 2 days PI only and in cloaca samples it was detected by virus isolation on 6 days PI. The virus was not detected in cecal tonsils, kidney, bursa of fabricius and thymus. Also there was not any mortality in any groups. Our results indicated that the H9N2 subtype of Avian Influenza in SPF chickens has limited dissemination, and perhaps other pathogens in commercial poultry houses cause extreme mortality and losses.

کلیدواژه ها:

Avian Influenza (H9N2) ، Virus Isolation ، Tissue dissemination ، SPF chickens