The Preventing Effects of Pentoxiphylin on Testis Histological Changes And Spermatogenesis Indexes In Mice Following Exposure To Sodium- Valproate

Backgournd: Sodium-Valproate (VPA) is commonly prescribed in the treatment of epilepsy and several kinds of seizures, causing oxidative stress. Pentoxiphylin (PX) as an antioxidantis used for treatment of muscle pain inpatient with peripheral artery disease and reduces oxidative stress. The aim of this study was to investigate the effect of pentoxiphylin on histological changes of testis tissue and spermatogenesis indexes in micetreated with sodium-valproate Method: 24 Adult male NMRI mice (36-2gr) were divided randomly into 4 groups (n=6): control, sodium valproate (500mg/kgi.p), sodium valproate+ pentoxiphylin (500 mg/kg i.p) andPentoxiphylin (100 mg/kgi.p). 14 days after treatment, the histology of the right testes were studied using stereological techniques. Data were analyzed using one way ANOVA and Tukey’s test and means were considered significantly different at P<0.05.Result: A significant decrease in the mean volume of testis, volume of seminiferous tubules,mean number of spermatocytes, round and long spermatids,Leydig andspermatogenesis indexes was found in the sodium valproate group compared to the control. The above parameters were increased in the sodium valproate +pentoxiphylingroupto the control level.The mean number of spermatogonia and Sertoli cells did not show a significant difference among all different groups.Conclusion: Our results indicated that pentoxiphylin, as an antioxidant,could reduce the adverse effects of sodium valproateon the Spermatogenesis indexes andchanges in the components of testis tissue.

کلیدواژه ها:

Stereology ، Pentoxiphylin ، spermatogenesis indexes ، sodium valproate ، Mice