Enhanced Viability of Cryopreserved Human Sperm Following Sericin Supplementation Of The Freezing Media

Background: Cryo-injuries in human sperm cryopreservation encouraged researchers to design a suitable protocol for sperm freezing. Supplementation of freezing media with antioxidant could be a suitable strategy to preserve the quality of sperm after cryopreservation. The objective was to determine the effects of various sericin concentrations (0, 0.5, 1, and 2.5 %) during freezing media on Human sperm viability.Methods: Human ejaculated spermatozoa were obtained by masturbation from either fertile man, capacitated spermatozoa were freezed using freezing media supplemented with 0, 0.5, 1 and 2.5% of sericin. Viability assessment before and after both freezing processing.Results: Addition of 2.5% of sericin during freezing processing significantly increased viability rates comparted with control (81.50 ± 12.29 and 62.50 ± 10.06, respectively). However, a too low concentration (0.5%) of sericin during freezing processing decreased viability rates compared with high concentrations (P<0.05).Conclusion: In conclusion, appropriate concentrations (2.5%) of sericin promoted human spermatozoa viability after thawing process in ART center.

کلیدواژه ها:

Sericin ، Human Spermatozoa ، Sperm Viability ، Sperm Freezing