Evaluating the association of CD82 gene polymorphism with bresat cancer in woman at Ardebil Province

bresat cancer is the second most common cancer and the second fatal cancer in women. Various factors, including environmental parameters, have certain effects on the cell cycle division process. The CD82 gene is a type III transmembrane protein from tetra-alpestine family playing a role in cellular connections. The expression of this gene in prostatic-derived cell lines is reduced compared with normal tissues.Methods: The statistical population consisted of 75 blocked and paraffinized bresat cancer samples and 60 healthy tissue samples obtained from the Iranian Tissue Bank. RNA was extracted using TRIzol™ precipitation method. Specific sequences were then amplified for both groups by RT-PCR. Afterward, specific regions were cut by RFLP technique.Results: Based on the results, 65% of the patient samples showed significant relationships with CD82 gene expression (P = 0.01, OR = 0.94, Cl = 0.61-2.23). The gene expression also increased with age to a certain degree in the patient group (P = 0.1, OR = 0.82, Cl = 0.60 – 1.98).Conclusion: The expression of CD82 gene in women was significantly correlated with bresat cancer compared to that of the control group. This result could indicate that the gene can be used as a biomarker for the identification of people at the risk of bresat cancer.

کلیدواژه ها:

Cancer ، bresat ، CD82 gene ، PCR ، polymorphism