Compare the thickness of the endometrium and height of endometrial epithelium in mice uterus before implantation in natural cycles followed by use of HMG - HCG drugs and epigallocatechin gallate

Background: Angiogenesis plays a major role in endometrial receptivity and thickening of the endometrium immediately before implantation. The aim of the present work was to evaluate the anti-angiogenic properties of epigallocatechin gallate (EGCG) from green tea in thickness of endometrium and height of endometrial epithelium.Methods: In this study, 40 adult female NMARI mice randomly divided into four groups. Control group received vehicle; HMG/HCG group received 7.5 IU HMG intraperitoneal (IP) and 48 h later 7.5 IU HCG was injected (IP) for ovarian stimulation; HMG/HCG + EGCG group received HMG and HCG in the same manner as previous group and also received 5 mg/kg EGCG at 0, 24, 48, and 72 h after injection of HMG; and the group EGCG received 5 mg/kg EGCG. A male mouse was kept with two female animals in the same cage for mating. Mice were dissected 96 h after administration of HMG (immediately before implantation) and tissue processing was carried out for the uterine specimens.Result: The thickness of the endometrium and height of endometrial epithelium decreased in receiving EGCG groups compared to the control and receiving HMG / HCG groups, although the difference was not significant (P > 0.05). The thickness of endometrium and height of endometrial epithelium were counted by Motic Images Plus 3.2 software under a light microscope.Conclusion: The use of gonadotropins and EGCG could not be significant changes in endometrial thickness of the endometrium and height of endometrial epithelium

کلیدواژه ها:

Epigallocatechin gallate ، Human menopausal gonadotropin / Human chorionic gonadotropin ، Implantation ، Angiogenesis