Bacteriospermia can affects semen parameters and sperm DNA fragmentation in normozoospermic males

Background: Several factors can alter semen quality and bacteriospermia could be one of them. Bacteriospermia can increase WBC and Ros level in semen samples and therefore cause sperm DNA damage. Sperm DNA damage is one of the male infertility indicators. The purpose of this study was the evaluation of any possible relation between the bacteriospermia and leukocytospermia with sperm DNA fragmentation.Methods: Ejaculated semen samples were collected from 40 men referred to Royan Institute. Samples were divided to two groups (leukocytospermia vs non-leukocytospermia ). Semen analysis was performed according to the World Health Organization (WHO) 2010 guidelines using CASA. The semen samples were cultured for a bacterial growth using standard bacteriological techniques. Sperm DNA fragmentation was examined by Sperm DNA Fragmentation Assay (SDFA) kit. Data analysis was carried out using IBM SPSS Statistics.Result: In this study, prevalence of 10 species bacteria was observed. The most common bacteria was Streptococcus viridians (42.1%) and the less frequently isolated organisms were Streptococcus agalactiae (2.6%) and E.coli (2.6%). Sperm motility and morphology were significantly lower in leukocytospermia group. Sperm DNA fragmentation was significantly higher in leukocytospermia group (pConclusion: Genital tract infections that are usually associated with leukocytospermia can negatively affect sperm parameters and increased levels of DNA fragmentation.

کلیدواژه ها:

DNA Fragmentation ، Semen culture ، Sperm ، Leukocytospermia