Genetic diversity of Iranian melon cultigens revealed by AFLP markers

سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 414

فایل این مقاله در 11 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJHST-2-1_005

تاریخ نمایه سازی: 21 فروردین 1397

چکیده مقاله:

Iran is a part of the secondary centre of origin of melons, and therefore, there is a wealth of genetic variation of these species in this country. The Amplified Fragment Length Polymorphism (AFLP) marker was applied to investigate the genetic variations among five major Iranian melon cultigens (Cucumis melo) and „Ananasi‟ as a general well-known cultivar. Ten primer pairs were used on 90 individuals producing 318 polymorphic fragments, with an average of 31.8 fragments per primer combination. The polymorphism rates ranged from 80 to 100%. The genetic similarities among accessions were calculated according to Dice‟s Similarity Index and used to construct a dendrogram based on the Unweighted Pair Group Method with Arithmetic Average (UPGMA). The genetic distance estimates based on AFLPs ranged from 0.29 to 0.63, with a mean of 47±0.3. Iranian melon genotypes and the „Ananasi‟ cultivar were considered as two separate groups on the cluster analysis. The principal coordinate analysis showed a separate allocation of the melon cultivar groups. The results demonstrated a wide diversity of Iranian melon cultigens. The high number of alleles and the high expected genetic diversity detected with the AFLP marker indicated that the Iranian melon cultigens had distinctive characteristics and were an important genetic diversity pool, which made them a valuable source of breeding materials

کلیدواژه ها:

Amplified fragment length polymorphis ، cucumis melo ، genetic distance ، molecular markers

نویسندگان

Mansoureh Danesh

Department of Horticulture, College of Abouraihan Campus, University of Tehran

Mahmoud Lotfi

Department of Horticulture, College of Abouraihan Campus, University of Tehran

Shiva Azizinia

Department of Horticulture, College of Abouraihan Campus, University of Tehran