Study of cytotoxicity properties of zn o nanoparticles ( nps) synthetized from brassica napus.l plant against human breast cancer cell lines mcf7

The cells of the living body are constantly growing, multiplying, and eventually destroyed after several times. In normal conditions, there is always a balance between the creation and removal of cells andwhen this balance is eliminated for any reason, various diseases are created such as cancer. breast cancer is one of the common cancers among women. Symptoms of this cancer include breast pain, an increase in size, modification in breast color and etc. Many factors , including nutrition, mutation and environment can be effective in developing cancer. Surgery, targeted therapy and chemotherapy areamong the commonplace treatments for cancer. Nowadays, nanotechnology has been provided an important treatment for cancer sick persons. The aim of the present study was investigating the effectsof Zn o (NPs) synthesized from Brassica Napus L by green methods on human breast cancer cells. MTT assay Initially MCF7 cell lines cultured in RPMI 1640 at 96-well plates. cells incubated for 24 h togrow and attach the floor of each well. afterward the cells treated with various concentrations of Zno (NPs) for 24, 48 and 72 h. after incubation period added 15 μl yellow tetrazolium salt solution to eachwell of the plate. Incubated for 4 hours at 37 c. Dissolved formazan crystalline to 100μl DMSO. cell viability measured by Elisa Reader. The cells were subjected to microscopic examination at differenttimes after treatment with nanoparticles to observe morphological changes. The cytotoxicity of Zn O NPs exhibited appropriate cytotoxic effects by decreasing the viability of cells to less than 50%compared to untreated cells at 24 hours at 7μg/ml concentration. The bioavailability of cells at a concentration of 10 μg/ml in 24 and 48 hours was about 40 %, while survival was reported at about 5%in 72 hours at a similar concentration. The results show that the survival rate of the cells significantly decreased in 72 hours after treatment compared with 24 and 48 hours. Morphological observationsrevealed variations in cell membrane shrinkage, cytoplasmic outgrowth and changes in cell size. The achieved result indicated the potential of Zn o (NPs) for use in the cancer therapy in future.

کلیدواژه ها:

Breast Cancer ، Cancer Prevention ، Cell and Cancer ، Cancer Treatment and Management ، Drugs and Cancer