Extraction of anthocyanins from saffron petals using high pressure CO2

High-pressure carbon dioxide extraction investigated as a green technology for the extraction of antioxidant compounds from saffron petals. A Box-Behnken design (BBD) was employed to optimizethe extraction temperature (40-80 ºC), extraction time (20-60 min), and extraction pressure (90-180bar). Optimization process using response surface methodology carried out and 3D response surfaces were plotted from the mathematical model. The optimum conditions of extraction were estimated to betemperature of 62 ºC, time of 47 min and pressure of 164 bar. Extraction using these optimizedconditions achieved the best TAC (3088 mg/100g). The experimental values agreed with those predicted, thus representing fitness of the model employed and the success of BBD in optimizing the extraction conditions

کلیدواژه ها:

Anthocyanin ، Antioxidant ، Box-Behnken ، Saffron petal