Evaluate the expression levels of miRNA124 and miRNA 130a in breast cancer metastasis tumors compared toprimary tumor

سال انتشار: 1394
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 516

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

این مقاله در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

NASTARANCANSER01_197

تاریخ نمایه سازی: 26 شهریور 1395

چکیده مقاله:

Breast cancer as a heterogeneous sophisticated disease includes several group withdiscrete clinical consequences. The disease is the most prevalent malignancy after nonmelanomaskin cancers and it is also considered as the second leading cause of death afterlung cancer. In fact, breast cancer is account for 23% of all cancer cases and 14% of deathsfrom cancer. The major cause of breast cancer deaths is actually metastasis of the tumor. Asa result, it is prominent to identify the disease mechanism and diagnose molecular tools inorder to predict metastasis. The aim of study is to analyze the expression of miRNA 124 andmiRNA 130 in primary tumor tissue and metastatic tissue of breast cancer. Therefore, asignificant difference between the desired expression of miRNA in primary tumor tissue andmetastatic breast cancer provides an opportunity to analyze diagnostic value of determiningplasma levels of miRNA as a non-invasive test for predicting metastasis. The specimenswere collected from 30 metastatic and 30 primary tumor tissues of breast cancer patients.After that, RNA extraction was accomplished by means of GeneAll kit and then was stored in-80 degrees. Then, cDNA synthesis was carried out by miscript II RT kit from Qiagenecompany. Finally, sybergreen Real Time PCR of all samples was done for miRNA124,miRNA130a and miRNA 16 as a reference by means of Pre-designed primers of Qiagenecompany.The results of molecular expression study showed that the amount of miRNA 124 inmetastatic tissues has approximately increased double of primary tumor tissues. It is alsorevealed that the amount of miRNA has similarly increased by about 1.7 times. According torecent results, it can be possible to regard molecule as a major cause of metastasis processin breast cancer.

کلیدواژه ها:

نویسندگان

Atieh Eslahi

Department of Genetics, Mashhad University of medical sciences, Mashhad, Iran

Mahdieh Daliri

Department of Genetics, Mashhad University of medical sciences, Mashhad, Iran

Majid Mojarrad

Department of Genetics, Mashhad University of medical sciences, Mashhad, Iran