Evaluation of c-Fos , LAT Expression and HTLV-I Viral Load in Adult T cell Lukemia/Lymphoma and HealthyCarriers

Human T-lymphotropic virus type 1 (HTLV-I) infects more than 20 million people worldwide. Itis associated with two human diseases; Adult T-cell Lukemia /lymphoma (ATLL) orHAM/TSP. ATLL as an aggressive leukemia/lymphoma is a malignancy with disregulation ofimmune resopnses, cell cycle and signaling pathways. LAT as an adaptor molecule in thecell signalling pathways and c-FOS as an important transcription factor in Immunity, cell cycleand cellular responses might be implicated in ATLL development, therefore the expression ofLAT and c-FOS have been evaluated in this study. The proviral load might be indicatingmonitoring markers for therapeutic efficiency of HTLV-I associated diseases anddevelopment. Eighteen HTLV-I healthy carriers and ninteen patients with ATLL wereassessed for LAT and c-FOS expression and proviral load of in PBMCs using real time PCR,TaqMan method. The data was analyzed by SPSS software. Interestingly, c-FOS did no anyassociation with ATLL development or progression, however, LAT expression and HTLV-Iproviral load was dramatically increased in ATLL patients compare to HTLV-I carriers (pvalue<0.000), and seems to be involved in HTLV-I associated ATLL progression. Resultsshowed that increased expression of LAT, as a master molecule of Tcell response mayinvolve in malignancy and TCR signalling may help to this reaction. The findings alsoshowed that c-FOS and MAPK pathway were not involved in ATLL development. Accordingto accumulated data increased HTLV-I proviral load is key to the process of leukemogenesisin HTLV-1 carriers

کلیدواژه ها:

HTLV-I ، Adult T-cell Lukemia /Lymphoma ، Proviral Load ، LAT ، c-Fos