Isolation and molecular Characterization of Bacillus cereus from Vitis vinifera in Iran

Vitis vinifera is one of the important economical plant species that can most reliably be produced in each area. Improving the quality and increasing resistance of grapevine to pathogen and unfavored environmental condition is essential. Chemical fertilizer was used to increase crop yields for several years that have negative impact on the environment. Sustainable agriculture emphasizes yield improvement without affecting environment; therefore application of endophytic bacteria is a way to sustainable agriculture. Endophytice bacteria promotes plant growth of different herbals by various process such as nitrogen fixation, solubilization of phosphate, production of phytohormones, sidrophore, posing antagonistic activity and induce high biotic and abiotic resistance for plants. Sustainable agriculture emphasizes yield improvement without negative affect on environment, Through the current study, the presence Bacillus cereus as endophytic bacteria was identified from Vitis vinifera in Iran by using 16SrDNA gene based PCR methods. The phylogenetic tree was drawn by MEGA 6 software using Neighbour-joining method and the significance of junctions was established using bootstrap method (1000 replicates). By further research about plant growth promoting feature of them, plant-based industries improved to achieve their commercial success and plant maintenance facilitate in sustainable ecosystems.

کلیدواژه ها:

Bacillus cereus ، Endophytice bacteria ، Vitis vinifera