Molecular Screening and Evaluation of Shiga Toxin ۲ Genes in Enterobacteriaceae Bacteria Isolated fromStool and Urine Specimens of the Patients Referred to the Falavarjan Therapeutic Clinic in Isfahan: ARapid Diagnostic Approach

Introduction: The investigation is continuing to determine virulence factors that may predict the severity andlong-term prognosis of Enterobacter infections. This information is paving the way for a future paradigm shift indiagnostic and therapeutic methods. Evaluating the diversity of Shiga toxins and the identification of theirstructures can provide us with targeted solutions to deal with pathogens. However, it is obvious that for targetedpresentations, more extensive research is needed to demonstrate their performance in future clinical applications.Methodology: In this study, Enterobacteriaceae strains isolated from stool and urine specimens of the patientsreferred to the falavarjan therapeutic clinic in isfahan were examined to detect the presence of the Shiga toxin ۲(Stx۲) gene. The isolates were cultured and analyzed through microbial and biochemical methods. To determinethe killer phenotype, an optimized well test was performed. The Stx۲ gene within the genomic DNA of theisolates was confirmed through colony PCR and real-time PCR. Finally, SDS-PAGE was employed to comparethe protein profiles of killer and non-killer bacterial strains.Discussion: A rapid molecular technique for Stx۲ gene screening was optimized in this study. Our assayconditions are confident and referable. We also purified, identified, and functionally examined the Stx۲ peptideand recorded the bacterial strains that produced the Stx۲ toxin.Conclusion: Real-time PCR tests, which are quick, easy, and accurate ways to find and screen forEnterobacteriaceae virulence genes, such as those from Stxs and other groups, can help doctors find and treatpatients more quickly.

کلیدواژه ها:

Molecular Screening ، Shiga Toxin ، Enterobacteriacea